An important role in the Ukrainian-Latvian relations belongs to the development of interregional cooperation. The legal basis of interregional cooperation consist of more than 20 documents, most important of which are: Agreement on cooperation between the capital of Latvia - Riga and the capital of Ukraine - Kyiv (29.05.1998); Agreement on cooperation between Cherkasy region and Daugavpils (14.09.2001); Protocol of intent on cooperation between Kirovograd region and Jelgava (18.09.2001); Agreement on cooperation between Kharkiv and Daugavpils (2006); Agreement on cooperation between Ivano-Frankivsk and Jelgava (24.10.2007); Agreement on institutional twinning between Lviv and Smiltene (11.05.2011); Agreement on institutional twinning between Mirgorod and Jekabpils (19.11.2014); Agreement on Cooperation between the Odessa Regional State Administration and the Development Council of the Vidzeme Planning Region of Latvia (May 21, 2019); Agreement on cooperation between Popasna state administration - military-civil administration and municipality of Ogre (22.05.2019).

Public-partnerships between the administrative-territorial units of the two countries are gradually expanding in the economic, cultural, humanitarian, scientific, educational and tourism spheres. There is an exchange of delegations at the self-government level. In particular, in 2019 the cooperation between the cities of Kyiv and Riga was resumed, new contacts were established between the resort cities of Truskavets and Jurmala, the issue of signing a twinning agreement between the cities of Bakhmut and Saldus was agreed.

Latvia provides active assistance in the rehabilitation of the Ukrainian children. In particular, in 2019, more than 120 Ukrainian children were able to recover their health in different regions of Latvia. For 2015-2019 with the support of local self-government bodies more than 350 children were on vacation in Latvia (in particular, whose parents are active members of the Ukrainian military, killed or injured due to war activities).